解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (1 ): 25-29.doi: 10.3969/j.issn.0529-1356.2013.01.005

• 神经生物学 • 上一篇    下一篇

二苯乙烯苷通过调节组蛋白去乙酰化酶1水平拮抗沙鼠脑缺血/再灌注损伤

陈晓宇1,王栋1,刘晓利1,陈晓蓉1,李俊2*   

  1. 1.安徽医科大学组织学胚胎学教研室; 2.药学院，合肥 230032
  • 收稿日期:2012-07-13 修回日期:2012-08-27 出版日期:2013-02-06 发布日期:2013-02-06
  • 通讯作者: 陈晓宇 E-mail:chenxiaoyuzlx@yahoo.com.cn
  • 基金资助:

    省自然科学基金资助项目;自然科学基金资助项目

Role of tetrahydroxy stilbene glucoside on histone deacetylase 1 level in cerebral ischemic-reperfusion injury in gerbils

CHEN Xiao-yu1,WANG Dong1,LIU Xiao-li1,CHEN Xiao-rong1,LI Jun 2*   

  1. 1.Department of Histology and Embryology; 2.School of Phamacy, Anhui Medical University, Hefei 230032, China
  • Received:2012-07-13 Revised:2012-08-27 Online:2013-02-06 Published:2013-02-06

摘要：

目的 探讨组蛋白去乙酰化酶1(HDAC1)水平改变在二苯乙烯苷（TSG）对沙鼠脑缺血/再灌注（I/R）损伤中的作用。方法 64只沙鼠随机分为假手术组、脑I/R模型对照组、TSG处理组和依达拉奉对照组。Morris水迷宫实验观察TSG对脑I/R鼠学习记忆功能的影响；神经功能评分、氯化三苯基四氮唑（TTC）染色观察TSG对经I/R脑组织的保护作用；免疫组织化学SP法观察脑组织中HDAC1蛋白的表达。Western blotting和RTPCR检测HDAC1蛋白和mRNA的水平。结果 与模型组比较，TSG对I/R鼠的脑组织有保护作用，能够改善模型鼠学习记忆能力，使模型鼠脑组织HDAC1蛋白和mRNA水平明显升高。 结论 TSG能够改善脑I/R损伤模型鼠认知功能，其作用机制与组蛋白乙酰化和去乙酰化动态平衡有关。

关键词: 脑缺血/再灌注损伤, 二苯乙烯苷, 组蛋白去乙酰化酶1, 免疫印迹法, 大鼠

Abstract:

bjective To investigate the role of tetrahydroxy stilbene glucoside(TSG) on histone deacetylase 1 (HDAC1) level in cerebral ischemic-reperfusion (I/R) injury in gerbils. Methods Sixty-four gerbils were randomly divided into four groups: sham group,model control group, TSG treatment group and the positive control medicine of edaravone injection group. The functions of learning and memory were detected by Morris water maze after 7 days. The cerebral infarction of protective action was investigated by nerve function score and triphenyltetrazolium chloride (TTC) staining. The protein and mRNA level of HDAC1 were detected by the immunohistochemical SP, Western blotting and RT-PCR methods. Results Compared with the model group, TSG had protective effect on ischemia reperfusion of the brain. TSG significantly enhanced the ability of learning and memory in I/R injury model gerbils. The protein expression and mRNA level of HDAC1 were significantly increased after TSG treatment in I/R injury model mice. Conclusion TSG has positively protective and therapeutic effect on cerebral I/R injury in gerbils. Its mechanism may be relevant with maintaining the homeostasis of histone acetylation and deacetylation.

Key words: Brain ischemia-reperfusion injury, Tetrahydroxy stilbene glucoside, Histone deacetylase 1, Western blotting, Rat